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KAKEN_16K18957seika.pdf
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:165.8 KB
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:Nov 12, 2018 |
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Total downloads since Nov 12, 2018 : 385
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| Title |
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新規白内障治療薬の開発を目指したアクアポリン0の機能解明
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シンキ ハクナイショウ チリョウヤク ノ カイハツ オ メザシタ アクアポリン 0 ノ キノウ カイメイ
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Shinki hakunaishō chiryōyaku no kaihatsu o mezashita akuaporin 0 no kinō kaimei
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Investigation of AQP0 functions for making anti-cataract drug that targets for AQP0
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中澤, 洋介
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ナカザワ, ヨウスケ
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Nakazawa, Yosuke
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慶應義塾大学・薬学部・助教
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Research team head
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科研費研究者番号 : 60411708
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2018
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科学研究費補助金研究成果報告書
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2017
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| Abstract |
本研究では, 水晶体特異的な水チャネルであるアクアポリン0(AQP0)の物質透過能および, 細胞接着能について検討した。
(1)物質透過能解析は, AQP0を恒常的に発現させたL細胞(L-AQP0)および, アフリカツメガエルの卵母細胞を用いて検討した。その結果, AQP0は水だけではなくアスコルビン酸を透過することが明らかとなった。また(2)細胞接着能解析の結果, L-AQP0とベクターコントロールあるいは, L-AQP0とAQP1安定発現L細胞の間で接着は認められなかったが, L-AQP0同士の組み合わせでのみ接着細胞が観察された。
(1) The permeation assay of AQP0 was used the stable L-cell line expressing AQP0. As the result, AQP0 can permeate ascorbic acid ex vivo, and also indicate that there is a difference between the import and export of ascorbic acid via AQP0 channel.
(2) The cell adhesion assay of AQP0 was revealed that AQP0 could bind directly to the opposing membrane through the C-loop domain. We also found that 109Pro and 110Pro were important amino acids for cell adhesion, but mutations in the C-loop of AQP0 did not affect AQP0 water permeability.
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研究種目 : 若手研究(B)
研究期間 : 2016～2017
課題番号 : 16K18957
研究分野 : 医療薬学, 生物学
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