| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_16K15448seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:978.7 KB
|
| Last updated |
:Sep 21, 2017 |
| Downloads |
: 264 |
Total downloads since Sep 21, 2017 : 264
|
|
| Release Date |
|
| Title |
| Title |
RNAウイルスを用いたiPS細胞治療用の未分化細胞除去ワクチンの開発
|
| Kana |
RNA ウイルス オ モチイタ iPS サイボウ チリョウヨウ ノ ミブンカ サイボウ ジョキョ ワクチン ノ カイハツ
|
| Romanization |
RNA uirusu o mochiita iPS saibo chiryoyo no mibunka saibo jokyo wakuchin no kaihatsu
|
|
| Other Title |
| Title |
Establishment of RNA virus vaccine for removal of pluripotent cells in iPS cell-derivative
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
福田, 恵一
 |
| Kana |
フクダ, ケイイチ
|
| Romanization |
Fukuda, Keiichi
|
| Affiliation |
慶應義塾大学・循環器内科・教授
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 20199227
|
| Name |
関, 倫久
|
| Kana |
セキ, トモヒサ
|
| Romanization |
Seki, Tomohisa
|
| Affiliation |
慶應義塾大学・救急科・助教
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 30528873
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2017
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2016
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
我々は, 拒絶免疫の誘導による未分化細胞除去法のProof of Conceptとして, ES細胞自体をマウスにワクチンすることで大量の同種マウスiPS細胞に対して奇形腫形成を抑制することが可能であることを確認した。マウス細胞を用いた同種移植のin vivoの奇形腫形成の実験系を確立するとともに, 既にワクチンによる免疫反応により奇形腫形成の抑制が可能であることのProof of Concept を得た。1x10^5オーダーの未分化細胞に対してはワクチンにより奇形腫形成が起こらなくなることを確認し, 未分化細胞の除去をin vivoで行うことの可能性を示した。
To establish of proof of concept of removal of pluripotent cells from induced pluripotent stem cell-derivatives, we tried to applied human embryonic stem cells to in vivo vaccination. In in vivo experiments of mouse assay, growth of teratoma which was derived from transplanted syngeneic mouse induced pluripotent stem cells were suppressed when mouse was vaccinated with irradiated human embryonic stem cells before transplantation. Additionally, vaccination with human embryonic stem cells completely inhibit teratoma formation when 1x10^5 syngeneic mouse induced pluripotent stem cells were transplanted. These results indicated that immune rejection against antigens which exists in pluripotent stem cells can be applicable to removal of pluripotent cells from induced pluripotent stem cell-derivatives in vivo.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 挑戦的萌芽研究
研究期間 : 2016～2016
課題番号 : 16K15448
研究分野 : 循環器内科
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
