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KAKEN_16K13091seika.pdf
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Download
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:217.5 KB
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| Last updated |
:Nov 12, 2018 |
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Total downloads since Nov 12, 2018 : 494
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有用海洋シアノバクテリアの培養株構築と生合成遺伝子の解析
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| Kana |
ユウヨウ カイヨウ シアノバクテリア ノ バイヨウカブ コウチク ト セイゴウセイ イデンシ ノ カイセキ
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Yūyō kaiyō shianobakuteria no baiyōkabu kōchiku to seigōsei idenshi no kaiseki
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Cultivation of useful marine cyanobacteria and analysis of biosynthetic genes
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末永, 聖武
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スエナガ, キヨタケ
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Suenaga, Kiyotake
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慶應義塾大学・理工学部・教授
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Research team head
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科研費研究者番号 : 60273215
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岩﨑, 有紘
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イワサキ, アリヒロ
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Iwasaki, Arihiro
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慶應義塾大学・理工学部・助教
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Research team member
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科研費研究者番号 : 00754897
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工藤, 史貴
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クドウ, フミタカ
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Kudo, Fumitaka
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東京工業大学・理工学部研究科・准教授
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Research team member
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科研費研究者番号 : 00361783
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2018
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科学研究費補助金研究成果報告書
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2017
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| Abstract |
カナミエナミドを産生する海洋シアノバクテリアMoorea bouilloniについて全ゲノム増幅を行った。吸光度・鎖長とも十分な質のゲノムサンプルが得られたことが確認できたので, 次世代シークエンサーによる網羅的配列解析を行った。バイオインフォマティクス的手法によりメチルエノールエーテル構造をもつ鎖状ペプチドlyngbyapeptin Bの生合成に関与すると思われる遺伝子を見出した。現在, モデル基質を用いた酵素反応と機能解析について検討している。また, カナミエナミドの全合成を達成し, その真の生物活性を明らかにした。
Whole genome amplification of marine cyanobacteria Moorea bouillonii which produces kanamienamide were conducted. Amplified genome DNA had good quality and purity, its sequence analysis was performed and biosynthetic gene clusters of lyngbyapeptin B with enol ether moiety were found. Enzymatic reaction with model substrates have been investigated. In addition, total synthesis of kanamienamide was achieved. The several assays of synthetic kanamienamide revealed its real biological activity
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研究種目 : 挑戦的萌芽研究
研究期間 : 2016～2017
課題番号 : 16K13091
研究分野 : 天然物化学
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