| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_16H02651seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:166.9 KB
|
| Last updated |
:Nov 14, 2022 |
| Downloads |
: 155 |
Total downloads since Nov 14, 2022 : 155
|
|
| Release Date |
|
| Title |
| Title |
クロマチンリモデリング因子による高品質iPS細胞の作製方法の確立
|
| Kana |
クロマチン リモデリング インシ ニ ヨル コウヒンシツ iPS サイボウ ノ サクセイ ホウホウ ノ カクリツ
|
| Romanization |
Kuromachin rimoderingu inshi ni yoru kōhinshitsu iPS saibō no sakusei hōhō no kakuritsu
|
|
| Other Title |
| Title |
The development of high quality iPS cells by chromatin remodeling factors
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
福田, 恵一
 |
| Kana |
フクダ, ケイイチ
|
| Romanization |
Fukuda, Keiichi
|
| Affiliation |
慶應義塾大学・医学部 (信濃町) ・教授
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 20199227
|
| Name |
湯浅, 慎介
|
| Kana |
ユアサ, シンスケ
|
| Romanization |
Yuasa, Shinsuke
|
| Affiliation |
慶應義塾大学・医学部 (信濃町) ・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 90398628
|
| Name |
國富, 晃
|
| Kana |
クニトミ, アキラ
|
| Romanization |
Kunitomi, Akira
|
| Affiliation |
慶應義塾大学・医学部 (信濃町) ・助教
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 30570882
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2020
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2019
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
人工多能性幹細胞 (iPS細胞)の質に関しては、染色体の安定性、遺伝子変異の有無、分化多能性に関して議論がなされてきた。またマウスES細胞並みの高品質ヒトiPS細胞として、naive iPS細胞に関する研究もなされてきた。本研究においては、再生医療の実現を念頭に置き、通常の体細胞からiPS細胞を作製する方法に導入する遺伝子を追加することにより、iPS細胞の品質向上を行ってきた。マウス体細胞においてレトロウイルスベクターを用いてiPS細胞樹立時にOct4、Sox2、Klf4と共にH1fooを強制発現させてiPS細胞を樹立し、同iPS細胞の高品質の確認を行ってきた。
In terms of the quality of iPS cells (induced pluripotent stem cells), there have been discussions regarding the chromosomal stability, inserted gene mutations, and pluripotency. In addition, research has been conducted on naive iPS cells as high-quality human iPS cells similar to mouse ES cells. In this study, for the realization of regenerative medicine, we have improved the quality of iPS cells by adding genes to the method for producing iPS cells from somatic cells. In mouse somatic cells, H1foo was introduced together with Oct4, Sox2, and Klf4 for the establishment of high quality iPS cells using a retrovirus vector, and the high quality of the iPS cells was confirmed.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 基盤研究 (A) (一般)
研究期間 : 2016～2018
課題番号 : 16H02651
研究分野 : 循環器
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
| Mar 5, 2021 | | インデックス を変更 |
| Nov 14, 2022 | | Creator Name,Creator Kana,Creator Romanization,Creator Affiliation,Creator Affiliation (Translated),Creator Role,Creator Link,Creator 著者ID,Abstract 内容,Note 注記 を変更 |
|
|
| Index |
|
| Related to |
|
