| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_15K20112seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:235.6 KB
|
| Last updated |
:Sep 21, 2017 |
| Downloads |
: 303 |
Total downloads since Sep 21, 2017 : 303
|
|
| Release Date |
|
| Title |
| Title |
尿路上皮癌におけるEZH2制御による抗腫瘍効果の検討
|
| Kana |
ニョウロ ジョウヒガン ニ オケル EZH2 セイギョ ニ ヨル コウシュヨウ コウカ ノ ケントウ
|
| Romanization |
Nyoro johigan ni okeru EZH2 seigyo ni yoru koshuyo koka no kento
|
|
| Other Title |
| Title |
Antitumor effect by regulation of EZH2 on urothelial carcinoma
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
早川, 望
|
| Kana |
ハヤカワ, ノゾミ
|
| Romanization |
Hayakawa, Nozomi
|
| Affiliation |
慶應義塾大学・医学部・助手
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 10528441
|
| Name |
菊地, 栄次
|
| Kana |
キクチ, エイジ
|
| Romanization |
Kikuchi, Eiji
|
| Affiliation |
慶應義塾大学・医学部・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
| Name |
宮嶋, 哲
|
| Kana |
ミヤジマ, アキラ
|
| Romanization |
Miyajima, Akira
|
| Affiliation |
慶應義塾大学・医学部・准教授
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
| Name |
大家, 基嗣
 |
| Kana |
オオヤ, モトツグ
|
| Romanization |
Oya, Mototsugu
|
| Affiliation |
慶應義塾大学・医学部・教授
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2017
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2016
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
EZH2の選択的メチル化抑制阻害剤である GSK126を用いて直接的な抗腫瘍効果の検討を行った。ウエスタンブロット法にて, ヒト膀胱癌細胞株(UMUC3, KU19-19, T24, 5637)におけるEZH2の発現は確認できたが, GSK126投与後のH3K27me3発現の変化は認められなかった。さらにWSTアッセイでは濃度・時間依存ともにGSK126の有効な殺細胞効果は認められなかった。EZH2抑制と免疫チェックポイント阻害との相乗効果が検討されているが, 上部尿路上皮癌検体においてPD-1発現を検討したところ, 腫瘍内部のPD-1強陽性が独立して上部尿路上皮癌死と関連していた。
We examined direct antitumor effect for urothelial carcinoma by using GSK126, which is an inhibitor of selective methylation of EZH2. Expression of EZH2 could be confirmed in human bladder cancer cell lines (UMUC3, KU19-19, T24, 5637) by the Western blotting method, whereas expression of H3K27me3 after administration of GSK126 in vitro was no change in the expression level compared with the control. Furthermore, we examined the effect of GSK on cell growth by WST assay, and no effective decrease of cell viability in either concentration dependence or time dependence. Recently, a synergistic effect between inhibition of EZH2 and immunocheckpoint inhibitor has been studied.We examined the role of PD-1 expression on the clinical outcome of upper tract urothelial carcinoma. PD-1 positivity in the tumor inside could be a strong predictor for a worse clinical outcome.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 若手研究(B)
研究期間 : 2015～2016
課題番号 : 15K20112
研究分野 : 泌尿器悪性腫瘍
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
