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KAKEN_15K14453seika.pdf
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:80.9 KB
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:Aug 1, 2019 |
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Total downloads since Aug 1, 2019 : 203
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| Title |
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ES細胞における減数分裂関連因子の機能解析
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| Kana |
ES サイボウ ニ オケル ゲンスウ ブンレツ カンレン インシ ノ キノウ カイセキ
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ES saibo ni okeru gensu bunretsu kanren inshi no kino kaiseki
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Functional analysis of meiosis-related factors in ES cells
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洪, 実
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コウ, ミノル
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Ko, Minoru
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| Affiliation |
慶應義塾大学・医学部・教授
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Research team head
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科研費研究者番号 : 50631199
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石黒, 啓一郎
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イシグロ, ケイイチロウ
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Ishiguro, Keiichiro
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慶應義塾大学・医学部・特任准教授
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Research team member
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科研費研究者番号 : 30508114
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洪, 繁
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コウ, シゲル
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Ko, Shigeru
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慶應義塾大学・医学部・准教授
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Research team member
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科研費研究者番号 : 90402578
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2017
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科学研究費補助金研究成果報告書
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2016
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| Abstract |
Zscan4はマウス着床前胚2細胞期に発現することが知られており, zygotic遺伝子群の活性化と減数分裂関連遺伝子の異所的な発現を特徴とする。本研究では内在性Zscan4c遺伝子座にGFPをノックインしたES細胞およびマウスを作製して, 内在性Zscan4の発現パターンの解析を行った。その結果, ES細胞内の内在性Zscan4cは全Zscan4陽性細胞のうち約30%であることが判明した。またmeiotic prophaseの後期に相当するGV oocyteおよびパキテン期の精母細胞でZscan4の発現が見られることが判明した。
Zscan4 is known to be expressed in the 2 cell stage mouse embryos and is characterized by activation of the zygotic genes and ectopic expression of meiosis-related genes. In this study, ES cells and mice in which GFP was knocked-in at the endogenous Zscan4c locus were prepared, and the expression pattern of endogenous Zscan4 was analyzed. As a result, it was found that the endogenous Zscan4c in ES cells is about 30% of all Zscan4 positive cells. Expression of Zscan4 was observed in GV oocytes corresponding to late stage of meiotic prophase and spermatocytes at Pachytene stage.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2015～2016
課題番号 : 15K14453
研究分野 : 発生生物学
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| Aug 1, 2019 | | 著者 を変更 |
| Aug 1, 2019 | | 著者 を変更 |
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