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KAKEN_15K11221seika.pdf
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Download
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:168.6 KB
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:Nov 12, 2018 |
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| Title |
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ゼノフリー治療用ヒトiPS細胞による霊長類歯周組織再生療法への展開
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ゼノフリー チリョウヨウ ヒト iPS サイボウ ニ ヨル レイチョウルイ シシュウ ソシキ サイセイ リョウホウ エノ テンカイ
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Zenofurī chiryōyō hito iPS saibō ni yoru reichōrui shishū soshiki saisei ryōhō eno tenkai
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Development for primate periodontal tissue regenerative therapy by human iPS cells for xeno-free system
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中川, 種昭
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ナカガワ, タネアキ
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Nakagawa, Taneaki
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慶應義塾大学・医学部(信濃町)・教授
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Research team head
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科研費研究者番号 : 00227745
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森川, 暁
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モリカワ, サトル
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Morikawa, Satoru
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慶應義塾大学・医学部(信濃町)・専任講師
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Research team member
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科研費研究者番号 : 00424169
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2018
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科学研究費補助金研究成果報告書
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2017
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本研究ではフィーダーフリー・ゼノフリーiPS培養法から, 歯周組織の起源である神経堤細胞への誘導を実現させた。マウスの歯根膜組織には神経堤細胞が存在し, その一部は間葉系幹細胞マーカーであるPDGFRαやCD44が発現していることを確認した。そのため神経堤由来間葉系幹細胞は歯周組織再生療法の有力な細胞供給源になる可能性が示唆された。これらの細胞を実際の歯周病モデルマウスに移植するべく, その疾患モデルマウスの作製とその評価を行った。マウスの大臼歯に絹糸を結紮することで骨破壊を誘導するというきわめて臨床の歯周病に近い歯周病誘発モデルの再現実験を行い, その技術的再現性を確認し移植実験の準備をしている。
In this study, we realized the induction of neural crest cells, which was feeder-free, xeno-free iPS culture method. It was confirmed that neural crest cells existed in the periodontal ligament tissue of the mouse and some of them expressed PDGFRα and CD44 which are mesenchymal stem cell markers. This suggested that neural crest derived mesenchymal stem cells may be a potential source of cells for periodontal tissue regeneration therapy. In order to transplant these cells into real periodontal disease model mice, the disease model mice were prepared and evaluated. Periodontal disease induction model induced bone destruction by ligating 5-0 silk thread to mouse molar tooth. To confirm the technical reproducibility. Now, we have been preparing for transplantation.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2015～2017
課題番号 : 15K11221
研究分野 : 歯周病学
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