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KAKEN_15K10727seika.pdf
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Download
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:372.1 KB
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Total downloads since Aug 30, 2019 : 647
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子宮体癌におけるDNAメチル化の網羅的解析とエピミューテーション
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シキュウタイガン ニ オケル DNA メチルカ ノ モウラテキ カイセキ ト エピミューテーション
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Shikyūtaigan ni okeru DNA mechiruka no mōrateki kaiseki to epimyūtēshon
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Genome-wide DNA methylation analysis in endometrial cancer
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矢野倉, 恵
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ヤノクラ, メグミ
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Yanokura, Megumi
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慶應義塾大学・医学部(信濃町)・特任助教
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科研費研究者番号 : 20433732
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阪埜, 浩司
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バンノ, コウジ
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Banno, Kōji
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慶應義塾大学・医学部(信濃町)・准教授
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Research team member
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科研費研究者番号 : 70265875
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2018
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科学研究費補助金研究成果報告書
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2017
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【目的】子宮体癌の中には, 様々な遺伝子に異常メチル化を認める癌が存在する。そこで今回我々は, 子宮体癌患者の末梢血DNAにおける異常メチル化をゲノムワイドに解析し, その特徴や原因を明らかにすることを目的とした。
【方法】倫理委員会の承認のもと, 子宮体癌患者25名の癌部DNAを解析し, メチル-high群とメチル(-)群に分類した。各群の末梢血DNAにおける遺伝子プロモーター領域のメチル化率を次世代シーケンサーにて解析・比較した。
【結果】メチル-high群末梢血DNAにおいて, miR-663aのプロモーター領域のメチル化率はメチル(-)群に比し, 有意に高値であった。
[Objective] Concurrent DNA methylation of multiple genes occurs in endometrial cancer. However, the features and causes of high-methylation rate endometrial cancer are not well understood. Therefore, the aim of this study was to investigate the DNA methylation status in normal tissue from patients with endometrial cancer.
[Methods] The study was approved by the Ethics Committee. The subjects were 25 patients with endometrial cancer from whom cancer tissue and peripheral blood cells (PBCs) were obtained. The 25 cancer samples were classified as Methyl-high, and Methyl-negative (Methyl (-)) by methylation-specific PCR (MSP) analysis of three genes. DNA libraries were prepared from peripheral blood DNAs from 2 Methyl-H and 2 Methyl (-) patients, and bisulfite sequencing was performed.
[Results] PBC DNA in Methyl-H cases had significant hypermethylation in the miR-663a promoter region, compared to Methyl (-) cases.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2015～2017
課題番号 : 15K10727
研究分野 : 婦人科腫瘍学
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| Nov 12, 2018 | | インデックス を変更 |
| Aug 30, 2019 | | 出版地 場所,出版者 名前,出版者 カナ,出版者 ローマ字,日付 出版年(to:yyyy),日付 作成日(yyyy-mm-dd),著者版フラグ を変更 |
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