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KAKEN_15K09724seika.pdf
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Download
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:463.8 KB
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:Aug 28, 2019 |
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脳性麻痺に対する自己羊水細胞由来幹細胞治療の開発
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ノウセイ マヒ ニ タイスル ジコ ヨウスイ サイボウ ユライ カンサイボウ チリョウ ノ カイハツ
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Nōsei mahi ni taisuru jiko yōsui saibō yurai kansaibō chiryō no kaihatsu
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Development of stem cell therapy derived from autologous amniotic fluid cells for cerebral palsy
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落合, 大吾
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オチアイ, ダイゴ
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Ochiai, Daigo
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慶應義塾大学・医学部(信濃町)・助教
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Research team head
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科研費研究者番号 : 80348713
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田中, 守
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タナカ, マモル
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Tanaka, Mamoru
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慶應義塾大学・医学部(信濃町)・教授
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Research team member
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科研費研究者番号 : 20207145
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2018
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科学研究費補助金研究成果報告書
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2017
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低酸素虚血性脳症(HIE)は, 脳性麻痺の主要因である。今回は,新生仔HIEモデルマウスを用いて,ヒト羊水幹細胞(hAFS) 投与の治療効果を検討した。ヒト羊水中CD117陽性細胞をhAFSとして分離した。生後9日目(P9)にマウスの右総頸動脈を結紮し, 8%酸素に30分間曝露させてHIEモデルを作成した。P19に治療群にはhAFSを, 非治療群にはPBSを鼻腔内投与した。P30に運動機能試験を実施し, 脳を組織標本として評価した。hAFS投与は, HIEモデルマウスの脳実質を回復し運動障害を軽減した。hAFS投与は, HIE慢性期の新規治療となる可能性が示唆された。
Hypoxic-ischemic encephalopathy (HIE) remains a major cause of cerebral palsy. However, the efficacy of human amniotic fluid stem cells (hAFS) for HIE, especially in the chronic phase, remains unclear. The aim of this study was to determine the effect of hAFS on the chronic phase of HIE.
hAFS were isolated from AF cells as CD117+ cells. Hypoxia-Ischemia (HI) was induced in mice at postnatal day 9 (P9). Animals intranasally received hAFS or PBS at P19 and were harvested for histological analysis after functional tests at P30. hAFS improved sensorimotor deficits in HIE by brain restoration followed by migration to the lesion. These results suggest that hAFS administration could be a novel treatment for HIE, especially in the chronic phase.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2015～2017
課題番号 : 15K09724
研究分野 : 再生医療
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| Nov 12, 2018 | | インデックス を変更 |
| Aug 28, 2019 | | 出版地 場所,出版者 名前,出版者 カナ,出版者 ローマ字,日付 出版年(to:yyyy),日付 作成日(yyyy-mm-dd),著者版フラグ を変更 |
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