iPS細胞からMyoR陽性を指標に, 腎臓組織幹細胞を分化誘導し, 腎臓組織幹細胞移植のCKDモデルにおける腎臓再生作用を検証した。具体的には, 腎臓組織からFACS分離したMyoR陽性細胞, iPS細胞より分化誘導したMyoR陽性細胞を３種類の慢性腎不全モデルに移植し, 腎機能や組織障害に対する再生作用を比較した。再現性の問題は残るものの, MyoR陽性細胞は腎臓機能の回復作用を持つことが示唆され, ヒトiPS細胞を用いた新規CKD治療法開発をめざす基礎データとなりうる成果と考えられる。
By using MyoR positivity as an index from iPS cells, kidney tissue stem cells were induced to differentiate, and kidney regenerating action in CKD model of kidney tissue stem cell transplantation was examined. Specifically, MyoR positive cells FACS isolated from kidney tissue, and MyoR positive cells differentiated from iPS cells were transplanted into three kinds of chronic renal failure models, and the renewal function and regeneration action against tissue damage were compared. Although the problem of reproducibility remains, MyoR positive cells are suggested to have a recovery action of kidney function, which is considered to be a fundamental data aiming at development of a novel CKD therapy method using human iPS cells.

研究種目 : 基盤研究(C)(一般)
研究期間 : 2015～2017
課題番号 : 15K09244
研究分野 : 再生医療