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PDF¥AN10079809-20090930-0001.pdf
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Download
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:914.5 KB
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| Last updated |
:Dec 16, 2009 |
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: 1707 |
Total downloads since Dec 16, 2009 : 1707
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| Title |
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Deposition onto Glass Surface of Two-dimensional Crystal Film of Streptavidin
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| Title |
ストレプトアビジン2次元結晶膜のガラス表面への転写固定
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ストレプトアビジン 2ジゲン ケッショウマク ノ ガラス ヒョウメン エノ テンシャ コテイ
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Sutoreputoabijin 2jigen kesshomaku no garasu hyomen eno tensha kotei
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| Creator |
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古野, 泰二
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| Kana |
フルノ, タイジ
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| Romanization |
Furuno, Taiji
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| Affiliation |
慶應義塾大学医学部物理学教室
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| Affiliation (Translated) |
Department of Physics, Hiyoshi Campus, Keio University School of Medicine
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慶應義塾大学日吉紀要刊行委員会
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| Kana |
ケイオウ ギジュク ダイガク ヒヨシ キヨウ カンコウ イインカイ
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| Romanization |
Keio gijuku daigaku Hiyoshi kiyo kanko iinkai
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2009
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慶應義塾大学日吉紀要. 自然科学
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The Hiyoshi review of the natural science
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46
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2009
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1
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11
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| Abstract |
The two-dimensional (2D) crystal film of streptavidin (SA) prepared at the air/water interface had been transferred onto a hydrophobic surface of silicon wafer and studied by atomic force microscopy1). In the present study the deposition of this film onto glass coverslip has been examined. Annealing the glass coverslip at 525 ºC rendered the surface smoother and stabilized adhesion of the transferred film. The use of glass as substrate for protein immobilization has the advantage that an optical microscope can be used in transmission mode, and the fluorescence quenching which generally occurs for dyes locating near metal or semiconductor surfaces can be avoided2）. Therefore, the 2D crystal film of SA deposited on glass would find wider applications than on silicon as the protein binding base using biotinylated proteins.
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| Table of contents |
1. Introduction
2. Experimental
2.1 Materials
2.2 Preparation and transfer of 2D crystal film of streptavidin
2.3 Thermal treatment of the glass coverslip
2.4 Atomic force microscopy
3. Results and Discussion
3.1 Surface roughness of glass coverslip, fused silica plate and silicon wafer
3.2 Deposition of PBLH/SA 2D crystal film onto thermally treated glass coverslip
3.3 Protein G/IgG assembly fabricated
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| Departmental Bulletin Paper
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