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KAKEN_26870575seika.pdf
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Download
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:368.9 KB
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Total downloads since Aug 2, 2019 : 266
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バクテリアのゲノムデザインに向けた、複製制御機構の開発
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バクテリア ノ ゲノム デザイン ニ ムケタ , フクセイ セイギョ キコウ ノ カイハツ
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Bakuteria no genomu dezain ni muketa, fukusei seigyo kiko no kaihatsu
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Artificially controlled replication machinery for bacterial genome design
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河野, 暢明
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コウノ, ノブアキ
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Kono, Nobuaki
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慶應義塾大学・大学院政策・メディア研究科・特任助教
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Research team head
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科研費研究者番号 : 90647356
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2016
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科学研究費補助金研究成果報告書
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2015
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本研究課題では, 環状染色体を持つバクテリアを対象とし, ゲノム対称構造と複製効率の関係性を解明することで, 人工的に制御された複製機構を維持したゲノムデザインを目指した。その結果, 情報学や遺伝学などにおける様々な技術を横断的に用い, ゲノム構造が複製挙動に与える影響を詳らかにし, ゲノム構造の観点からバクテリアゲノムの進化を議論する事で, 複製効率を視野に入れた人工ゲノムのデザインへの足がかりを築き上げる事に成功した。
In this project, about a bacteria harboring circular chromosome, we set a goal to recognize the relationship between the genomic structure and replication efficiency, and design a bacterial genome by using various approaches in bioinformatics and genetics. As the results, first, we succeeded in constructing various mutants whose genomic structures were disrupted by inversion and dissection mutations in Bacillus subtilis, and developed a protocol for replication profiling method to observe the behavior of replication forks in cell. Using the mutants and methods, we cleared that the treatment of some gene factor enables to control the replication machinery.
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研究種目 : 若手研究(B)
研究期間 : 2014～2015
課題番号 : 26870575
研究分野 : 合成生物学
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| Aug 2, 2019 | | 著者,抄録 内容,著者版フラグ を変更 |
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