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KAKEN_26640132seika.pdf
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Title |
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高速・高精細三次元1分子測定システムの開発
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コウソク・コウセイサイ サンジゲン 1ブンシ ソクテイ システム ノ カイハツ
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Kosoku koseisai sanjigen 1bunshi sokutei shisutemu no kaihatsu
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Development of high-speed, high-resolution 3D single-molecule measurement system
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舟橋, 啓
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フナハシ, アキラ
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Funahashi, Akira
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慶應義塾大学・理工学部・生命情報学科・准教授
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Research team head
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科研費研究者番号 : 70324548
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広井, 賀子
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ヒロイ, ノリコ
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Hiroi, Noriko
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慶應義塾大学・理工学部・生命情報学科・専任講師
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Research team member
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科研費研究者番号 : 20548408
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2016
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科学研究費補助金研究成果報告書
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2015
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細胞分裂過程における微小管動態の制御機構は未だ明らかにされていない点が多く, これを明らかにする上で3次元的な微小管動態を定量的に評価することが重要である。しかし, 現行の顕微鏡システムでは焦点移動における技術的な問題から分裂期微小管動態の解析が困難であった。本研究課題では電気式焦点可変レンズ(ETL)を顕微鏡に組み込むことで分裂期微小管動態の3次元解析を可能とするトラッキングシステムを構築し, 分裂期微小管動態の3次元トラッキングを行い, 平均速度及びlifetimeを算出した。今後の展望として, 長時間観察を可能とする実験系の構築, 微小管動態の観測による紡錘体結合タンパク質の機能解析が挙げられる。
Although cell division is a well-known physiological event and historical target of biological research, the molecular mechanisms remains poorly understood area of biology. Quantification of the microtubule dynamics in 3D is a key to reveal the mechanisms. However, due to the physical constraints of samples or the microscope objective in the 3D, conventional microscopes are not suitable for 3D analysis of microtubules. We solved this problem by attaching Electrically tunable lens (ETL) to the microscope and using the ETL as a focusing device. By using our system, we measured the microtubule dynamics in mitosis and acquired quantitative data. The mean value of growth speed at 3D was statistically faster than the 2D result, on the other hand, there was no significant difference between the lifetime of at 3D analysis and 2D analysis because of the quick bleach of fluorescent proteins. We are planning to improve the experimental method to achieve long time analysis of the microtubules.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2014~2015
課題番号 : 26640132
研究分野 : 定量生物学
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