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KAKEN_25670754seika.pdf
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Download
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:83.4 KB
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Total downloads since Aug 1, 2019 : 470
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| Title |
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非接着培養で形成される凝集塊の未分化性獲得機構の解明
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| Kana |
ヒセッチャク バイヨウ デ ケイセイサレル ギョウシュウカイ ノ ミブンカセイ カクトク キコウ ノ カイメイ
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Hisetchaku baiyo de keiseisareru gyoshukai no mibunkasei kakutoku kiko no kaimei
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The mechanism to obtain undifferentiated ability by making aggregate
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貴志, 和生
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キシ, カズオ
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Kishi, Kazuo
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慶應義塾大学・医学部・教授
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Research team head
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科研費研究者番号 : 40224919
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荒牧, 典子
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アラマキ, ノリコ
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Aramaki, Noriko
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慶應義塾大学・医学部・講師
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Research team member
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科研費研究者番号 : 80365311
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林, 瑠加
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ハヤシ, ルカ
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Hayashi, Ruka
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慶應義塾大学・医学部・助教
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Research team member
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科研費研究者番号 : 50445392
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2015
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科学研究費補助金研究成果報告書
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2014
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| Abstract |
細胞凝集塊形成による未分化性獲得のメカニズムに迫ることを目的とした。マウス線維芽細胞凝集塊形成により, 未分化マーカーの上昇を認めた。しかしこれらは, 作成したクローンの中でバラツキがあり, 単一の細胞から分裂した線維芽細胞であっても, 細胞塊を形成する段階で, 周囲環境の違いにより, さまざまな未分化マーカーの発現の差異を生じることが判明した。
This study aimed to find out the mechanism of getting undifferentiated ability by making aggregates. In mouse fibroblasts, undifferentiated markers were upregulated by making aggregates. The levels of undifferentiated markers were, however, differs in between each clones. Surrounding conditions were thought to affect the expression levels of these undifferentiated markers.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2013～2014
課題番号 : 25670754
研究分野 : 形成外科
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