Item Type |
Article |
ID |
|
Preview |
Image |
|
Caption |
|
|
Full text |
KAKEN_25462608seika.pdf
Type |
:application/pdf |
Download
|
Size |
:394.0 KB
|
Last updated |
:Jan 6, 2017 |
Downloads |
: 304 |
Total downloads since Jan 6, 2017 : 304
|
|
Release Date |
|
Title |
Title |
癌抑制型microRNAを用いた子宮体癌治療 : 抗癌剤感受性増強とExocure
|
Kana |
ガン ヨクセイガタ microRNA オ モチイタ シキュウタイガン チリョウ : コウガンザイ カンジュセイ ゾウキョウ ト Exocure
|
Romanization |
Gan yokuseigata microRNA o mochiita shikyutaigan chiryo : koganzai kanjusei zokyo to Exocure
|
|
Other Title |
Title |
Endometrial cancer treatment by tumor suppressor microRNA
|
Kana |
|
Romanization |
|
|
Creator |
Name |
阪埜, 浩司
|
Kana |
バンノ, コウジ
|
Romanization |
Banno, Koji
|
Affiliation |
慶應義塾大学・医学部・専任講師
|
Affiliation (Translated) |
|
Role |
Research team head
|
Link |
科研費研究者番号 : 70265875
|
|
Edition |
|
Place |
|
Publisher |
|
Date |
Issued (from:yyyy) |
2016
|
Issued (to:yyyy) |
|
Created (yyyy-mm-dd) |
|
Updated (yyyy-mm-dd) |
|
Captured (yyyy-mm-dd) |
|
|
Physical description |
|
Source Title |
Name |
科学研究費補助金研究成果報告書
|
Name (Translated) |
|
Volume |
|
Issue |
|
Year |
2015
|
Month |
|
Start page |
|
End page |
|
|
ISSN |
|
ISBN |
|
DOI |
|
URI |
|
JaLCDOI |
|
NII Article ID |
|
Ichushi ID |
|
Other ID |
|
Doctoral dissertation |
Dissertation Number |
|
Date of granted |
|
Degree name |
|
Degree grantor |
|
|
Abstract |
【目的】子宮体癌においてエピジェネティックに発現抑制される癌抑制型microRNAを同定し, microRNAの効果および抗癌剤との併用による抗腫瘍効果を検討する。
【結果】4種の子宮体癌細胞株に対し脱メチル化処理を行った結果, 全ての細胞株に共通して発現上昇するmicroRNAとしてmiR-34bを同定した。また, 子宮体癌細胞株にmiR-34bを導入することにより, コロニー形成能や細胞遊走能の低下が認められた(p<0.05)。さらに, HEC-1Bをヌードマウスの皮下に移植し各薬剤を投与した。その結果, パクリタキセル+miR-34b群は他の群に比し有意に腫瘍径の縮小が認められた(p<0.05)。
[Objective] microRNAs have key roles in the onset, development and drug resistance of cancer. We identified miR-34b as a tumor suppressor-type microRNA with expression that is epigenetically suppressed in endometrial cancer. In this study, the antitumor effect of combined miR-34b and antitumor drugs on endometrial cancer was examined.
[Results] There was no change in cell viability after administration of miR-34b following application of cisplatin or adriamycin to HEC-108, HEC-1B and KLE cells. However, after treatment with paclitaxel, cell viability after administration of miR-34b decreased in all three cell lines. Tumor development occurred 14 days after transplantation of HEC-1B cells in nude mice. Drugs were administered on that day and thereafter. The tumor diameter significantly decreased in mice treated with paclitaxel + miR-34b compared with the results for other treatments (p<0.05).
|
|
Table of contents |
|
Keyword |
|
NDC |
|
Note |
研究種目 : 基盤研究(C)(一般)
研究期間 : 2013~2015
課題番号 : 25462608
研究分野 : 婦人科腫瘍学
|
|
Language |
|
Type of resource |
|
Genre |
|
Text version |
|
Related DOI |
|
Access conditions |
|
Last modified date |
|
Creation date |
|
Registerd by |
|
History |
|
Index |
|
Related to |
|