| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_24225001seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:1.7 MB
|
| Last updated |
:Sep 21, 2017 |
| Downloads |
: 666 |
Total downloads since Sep 21, 2017 : 666
|
|
| Release Date |
|
| Title |
| Title |
革新的高輝度近赤外発光プローブの創製と生体内癌イメージングへの応用
|
| Kana |
カクシンテキ コウキド キンセキガイ ハッコウ プローブ ノ ソウセイ ト セイタイナイガン イメージング エノ オウヨウ
|
| Romanization |
Kakushinteki kokido kinsekigai hakko purobu no sosei to seitainaigan imejingu eno oyo
|
|
| Other Title |
| Title |
Creation of bright near-infrared bioluminescent probes and their application to tumor imaging
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
鈴木, 孝治
 |
| Kana |
スズキ, コウジ
|
| Romanization |
Suzuki, Koji
|
| Affiliation |
慶應義塾大学・理工学部・教授
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 80154540
|
| Name |
佐藤, 守俊
|
| Kana |
サトウ, モリトシ
|
| Romanization |
Sato, Moritoshi
|
| Affiliation |
東京大学・大学院総合文化研究科・准教授
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 00323501
|
| Name |
金, 誠培
|
| Kana |
|
| Romanization |
Kim, Sung-Bae
|
| Affiliation |
国立研究開発法人産業技術総合研究所・環境管理研究部門・主任研究員
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 60470043
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2017
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2016
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
本研究では世界標準となる人工生物発光イメージングプローブ作製を目標に, 合成発光基質分子と遺伝子工学的手法に基づくタンパク質変異体とから独創的な"融合型人工生物発光分子プローブ"開発を行った。開発したアルキル化CTZ誘導体(6-Al3OH-CTZ)は, 酵素RLuc8.6-535を用いると市販基質DeepBlueCTMの約50倍高輝度の発光バイオイメージングを実現した。また, 分子歪みセンサーと呼ぶ人工酵素系発光プローブは発光酵素タンパク質に物理的歪みを加えることにより発光輝度の増減を制御でき, 従来のスプリット型生物発光プローブの約100倍の強い発光輝度を示す高感度バイオセンサーの開発ができた。
Bioluminescence is emitted by an enzymatic reaction involving a bioluminescent substrate luciferin and an enzyme luciferase. One of luciferases, Renilla (RLuc), generates cofactor-free bioluminescence with native coelenterazine (nCTZ). The design of novel CTZ derivatives resulting in enhanced optical intensity with prolonged bioluminescence is challenging. Our developed novel CTZ derivative with extension at the C-6 position of the imidazopyrazinone show significant bioluminescence emission with RLuc variant (RLuc8.6-535). This CTZ derivative exhibited about 50 times higher than that of DeepBlueCTM. Optical bioimaging of protein-protein interactions (PPIs) facilitates comprehensive elucidation of intracellular molecular events. We developed an optical measure for visualizing molecular tension triggered by any PPI named "a combinational probe". This type of probe expands capabilities of luciferases as a tool for analyses of molecular dynamics and cell signaling in living subjects.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 基盤研究(S)
研究期間 : 2012～2016
課題番号 : 24225001
研究分野 : 分析化学
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
