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KAKEN_18K09120seika.pdf
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:83.4 KB
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:May 17, 2022 |
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力学的負荷誘発性in vitro OA modelの確立と関節破壊様式の解析
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| Kana |
リキガクテキ フカ ユウハツセイ in vitro OA model ノ カクリツ ト カンセツ ハカイ ヨウシキ ノ カイセキ
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Rikigakuteki fuka yūhatsusei in vitro OA model no kakuritsu to kansetsu hakai yōshiki no kaiseki
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Establishment of mechanical load-induced OA model and analysis of articular cartilage destruction mode
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二木, 康夫
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ニキ, ヤスオ
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Niki, Yasuo
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慶應義塾大学・医学部 (信濃町) ・准教授
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Research team head
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科研費研究者番号 : 10276298
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2021
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科学研究費補助金研究成果報告書
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2020
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| Abstract |
in vitro OA modelの確立を目標とし、マウスの大腿骨に対して三次元力学的刺激装置を用いて圧負荷および各種試薬（IL-1β、ヒアルロン酸、Kartogeninなど）を加えながら器官培養を行った。real time PCRおよび組織切片の免疫染色を行ったところ、メカニカルストレスが各種炎症性サイトカインシグナルおよび関節軟骨破壊を増強する傾向を認めた一方で、新規治療薬候補として関節軟骨保護作用が期待されるヒアルロン酸やKartogeninを添加しても関節軟骨破壊の明らかな抑制には至らなかった。
For the purpose of establishing a new in vitro OA model, we performed organ culture on the femur of mice while applying mechanical stress loading and various reagents (IL-1β, hyaluronic acid, Kartogenin, etc.) using a three-dimensional mechanical stimulator.Real time PCR and immunostaining of tissue sections showed that mechanical stress tended to enhance various inflammatory cytokine signals and articular cartilage destruction.On the other hand, the addition of hyaluronic acid and Kartogenin, which are expected to have a protective effect on articular cartilage as new therapeutic drug candidates, did not clearly suppress the destruction of articular cartilage.
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研究種目 : 基盤研究 (C) (一般)
研究期間 : 2018～2020
課題番号 : 18K09120
研究分野 : 人工関節、軟骨再生、スポーツ医学
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