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KAKEN_17K01369seika.pdf
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細胞配置と力学的刺激の複合効果がもたらす毛髪および皮膚附属器官の完全生体外再生
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サイボウ ハイチ ト リキガクテキ シゲキ ノ フクゴウ コウカ ガ モタラス モウハツ オヨビ ヒフ フゾク キカン ノ カンゼン セイタイガイ サイセイ
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Saibō haichi to rikigakuteki shigeki no fukugō kōka ga motarasu mōhatsu oyobi hifu fuzoku kikan no kanzen seitaigai saisei
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Fully in vitro process for hair follicle regeneration using cell organization and mechanical stimulation
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宮田, 昌悟
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ミヤタ, ショウゴ
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Miyata, Shōgo
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慶應義塾大学・理工学部 (矢上) ・准教授
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Research team head
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科研費研究者番号 : 70376515
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2020
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科学研究費補助金研究成果報告書
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2019
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本研究では毛包組織構造と力学的環境を再現することで生体外プロセスのみで毛包と立毛筋に代表される皮膚付属器官を再生することを目的とした.
毛包の再生では,マウス胎児由来表皮細胞,ES細胞,サイトカイン層の三層構造からなるマイクロゲルビーズを用いて毛包構造を再現した.これより,生体外培養のみで毛包様構造の再構築を実現した.
さらに,立毛筋の構築のために筋芽細胞株を含有するマイクロゲルファイバを構築し,皮膚が通常さらされる力学的環境を模擬した引張刺激を印加する培養デバイスを開発した.力学的刺激を印加することで微細な筋管様組織の形成を認めた.これは立毛筋を再現する培養手法として有効であると考えられた.
The purpose of this study is to regenerate hair follicle and cutaneous appendages by only in vitro process by simulating in vivo cellular organization and mechanical condition.
Three-layered gel micro-bead culture containing fetal mouse epidermal cells, mouse ES cells and cytokines was established to simulate “native” hair follicle structure. By control of the cellular organization, and the cytokine gradient from the core to outer shell of the gel beads, hair follicle-like structures were reconstructed during the in vitro culture.
C2C12 cells, a skeletal muscle myoblast cell line, were used to study the effect of cellular orientation and cyclic mechanical stimuli on the regeneration of erector muscle of hair. To control cellular orientation, the cells were contained in gel fibers and stretched cyclically by custom-made cell-culture device. As the results, the directions of myotubes were oriented and the micro-scale mature myotubes could be formed to simulate the erector muscle of hair.
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研究種目 : 基盤研究 (C) (一般)
研究期間 : 2017~2019
課題番号 : 17K01369
研究分野 : 再生医工学
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