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KAKEN_16K01937seika.pdf
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| Title |
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新規質量分析イメージングによるパースルフィド依存性虚血再灌流障害制御機構の解明
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シンキ シツリョウ ブンセキ イメージング ニ ヨル パースルフィド イゾンセイ キョケツ サイカンリュウ ショウガイ セイギョ キコウ ノ カイメイ
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Shinki shitsuryō bunseki imējingu ni yoru pāsurufido izonsei kyoketsu saikanryū shōgai seigyo kikō no kaimei
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Functional analyses of sulfur-containing amino acid-derived thiol compounds in the ischemic reperfusion disorder by MALDI MS imaging
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菱木, 貴子
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ヒシキ, タカコ
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Hishiki, Takako
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慶應義塾大学・医学部 (信濃町)・講師
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Research team head
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科研費研究者番号 : 10338022
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2019
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科学研究費補助金研究成果報告書
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2018
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「活性含硫化合物 (分子内に-SH基あるいは-SSH基を持つ化合物) 」と虚血再灌流障害時の酸化ストレス軽減機能との関連について、質量分析技術を用いて検証を行った結果、すでにその抗酸化作用が知られている硫化水素のみならず、ホモシステイン、システイン、グルタチオン、そしてそれらの分子にさらに過剰な硫黄原子 (S) が結合したパースルフィド化合物が虚血再灌流障害の軽減に効果があることがわかった。さらに、組織中のパースルフィド化合物を「空間的情報」を保持したまま可視化する手法として新たにSERS (Surface-enhanced Raman spectroscopy) imaging法の確立に成功した。
The aim of this study is to be clarified the mechanisms that the thiol (containing -SH group) and persulfide (containing -SSH group that is one of the most powerful anti-oxidant thiol compounds) species could reduce intracellular oxidative stresses in the ischemic diseases. First, we performed measurements of the thiol and persulfide in the ischemic mice heart, suggesting that many thiol-containing metabolites play important roles to prevent from heart failure. Besides quantifications of thiol-compounds, we tried the MALDI (Matrix-Assisted Laser/Desorption Ionization) MS imaging for the visualization of the thiol and persulfide on the tissue sections. However, MALDI-MS could oxidize the thiol group easily, making us underestimate the contents of thiol compound on the tissue. Therefore, we apply another method, SERS (Surface-enhanced Raman spectroscopy) imaging technology. SERS enabled us to visualize the tissue distribution of the thiol and persulfide without labelling or staining.
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研究種目 : 基盤研究 (C) (一般)
研究期間 : 2016～2018
課題番号 : 16K01937
研究分野 : 代謝生化学
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