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KAKEN_16H02218seika.pdf
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Title |
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コヒーレントX線回折による酵母核内の核酸分布イメージング
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Kana |
コヒーレント Xセン カイセツ ニ ヨル コウボ カクナイ ノ カクサン ブンプ イメージング
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Kohīrento Xsen kaisetsu ni yoru kōbo kakunai no kakusan bunpu imējingu
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Visualization on the distribution of nucreic acids in yeast nucleus by using coherent X-ray diffraction
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中迫, 雅由
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ナカサコ, マサヨシ
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Nakasako, Masayoshi
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慶應義塾大学・理工学部 (矢上) ・教授
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Research team head
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科研費研究者番号 : 30227764
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2019
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科学研究費補助金研究成果報告書
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2018
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シンクロトロン放射光を用いた低温X線回折イメージング・トモグラフィーと、X線自由電子レーザーを用いた低温X線回折イメージングにおける試料作製および実験装置の高度化をおこなうとともに、新たな構造解析法を考案した。それらをもとに、バクテリア細胞や酵母細胞核の普遍的な内部構造などを可視化するとともに、バクテリア細胞や酵母細胞の三次元構造解析を行い、生体非結晶粒子の非侵襲イメージングを確立した。
In this project, we improved fundamental techniques including automatic control procedures of diffraction apparatuses, preparation of frozen-hydrated biological specimens, and measurement of spatial coherence of X-ray beam, in cryogenic X-ray diffraction imaging tomography using synchrotron X-rays and cryogenic X-ray diffraction using X-ray free electron laser pulses. In addition, we developed methods to obtain reliable electron density maps in phase retrieval calculation in structure analyses including the utilization of manifold learning. Based on these developments, we successfully established X-ray diffraction imaging technique for non-crystalline biological specimens, such as cells and organelles, through the visualization of common structures in yeast nucleus and cyanobacterium cell as well as the three-dimensional structures of yeast and bacteria cells without sectioning and chemical labeling necessary in other imaging techniques.
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研究種目 : 基盤研究 (A) (一般)
研究期間 : 2016~2018
課題番号 : 16H02218
研究分野 : 生物物理
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