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KAKEN_15K20285seika.pdf
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Download
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:220.4 KB
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:Sep 21, 2017 |
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Total downloads since Sep 21, 2017 : 232
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| Title |
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培養上皮内における未分化細胞と増殖秩序
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| Kana |
バイヨウ ジョウヒナイ ニ オケル ミブンカ サイボウ ト ゾウショク チツジョ
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Baiyo johinai ni okeru mibunka saibo to zoshoku chitsujo
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Label retaining cells in long term cultured confluent epithelial cell sheets
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庭野, 博子
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ニワノ, ヒロコ
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Niwano, Hiroko
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| Affiliation |
慶應義塾大学・医学部・特任研究員
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Research team head
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科研費研究者番号 : 90748518
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榛村, 重人
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シンムラ, シゲト
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Shinmura, Shigeto
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Research team member
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宮下, 英之
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ミヤシタ, ヒデユキ
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Miyashita, Hideyuki
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Research team member
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2017
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科学研究費補助金研究成果報告書
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2016
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| Abstract |
再生医療にも用いられる培養上皮中に, 生体と同様の, ゆっくり増殖する未分化細胞が存在するか検討した。コンフルエント直前の初代培養角膜輪部上皮細胞にEdUを3日間連続投与後, コンフルエントのまま6ヶ月間培養し, ゆっくり増殖する細胞をLabel retaining cells(LRCs)として検出した。EdU陽性率は標識直後の58.8%から追跡後は8.8%まで有意に減少したが, 未分化細胞マーカーK15陽性細胞塊では34.8%, K15陰性細胞では6.0%で, K15陽性細胞で高い傾向にあるものの有意差は認められなかった(p=0.09)。結果は研究協力者の論文で発表した。
To confirm whether the cultured epithelial sheets contain slow cycling cells as like in vivo tissue, the researcher labeled proliferating cells in limbal epithelial cells around confluence by serial 3 day feeding of EdU, and cultured confluent sheets for additional 6 months. Although EdU rate was significantly decreased from 58.8% to 8.8%, EdU rate in undifferentiated epithelial cells (K15+ cell clusters) was 34.8%, was not significantly different from the rete 6.0% in K15 negative differentiated cells (p=0.09), due to its high variance. We published our data including this study in Scientific Reports on 2017, 10.1038/srep43557.
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研究種目 : 若手研究(B)
研究期間 : 2015～2016
課題番号 : 15K20285
研究分野 : 眼科
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