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KAKEN_15K10730seika.pdf
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Title |
Title |
卵巣癌細胞の薬剤感受性と遺伝子プロファイルに基づいた治療法の確立
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Kana |
ランソウガン サイボウ ノ ヤクザイ カンジュセイ ト イデンシ プロファイル ニ モトズイタ チリョウホウ ノ カクリツ
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Romanization |
Ransōgan saibō no yakuzai kanjusei to idenshi purofairu ni motozuita chiryōhō no kakuritsu
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Towards precision medicine for ovarian cancer using drug sensitivity and resistance testing and genomic analysis
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冨永, 英一郎
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トミナガ, エイイチロウ
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Tominaga, Eiichiro
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慶應義塾大学・医学部(信濃町)・講師
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Research team head
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科研費研究者番号 : 80276328
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平沢, 晃
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ヒラサワ, アキラ
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Hirasawa, Akira
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慶應義塾大学・医学部(信濃町)・講師
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Research team member
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科研費研究者番号 : 90296658
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赤羽, 智子
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アカハネ, トモコ
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Akahane, Tomoko
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慶應義塾大学・医学部(信濃町)・特任助教
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Research team member
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科研費研究者番号 : 40398699
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2018
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科学研究費補助金研究成果報告書
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2017
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Abstract |
本研究課題内において以下のような成果を挙げた。
①患者腹水検体より腹膜中皮腫細胞株を樹立することに成功した。②樹立細胞株を使った2次元および3次元培養下での薬剤感受性試験を実施した。悪性中皮腫標準治療に使用される薬剤ペメトレキセドは2次元培養下では薬剤感受性があったのに対し, 3次元培養下では薬剤効果はなかった。結果は臨床経過と一致し生体内環境を模倣した薬剤感受性試験の実施が可能であった。③卵巣癌患者の腫瘍病変組織片より, スフェロイドを形成することを可能とし生体内構造に類似する腺管構造を作り出すことに成功した。卵巣癌腫瘍組織由来のスフェロイド形成に成功したのは本研究が初であり有用な成果となった。
The following results were raised within this research project.
①We successfully established a new cell line from malignant mesothelioma ascites. This cell line is a valuable biological resource to study the mechanisms underlying carcinogenesis in peritoneal mesothelioma and for drug sensitivity tests (DSRT). ②DSRT were conducted in two dimensional and three dimensional cultures using established cell lines. The pemetrexed (PEM) is used for standard therapy of malignant mesothelioma. PEM was drug susceptibility in two dimensional culture by DSRT, but there was no drug effect in three dimensional culture. Those results were consistent with the clinical course and it was possible to conduct a DSRT that mimics the in vivo environment. ③Cell cultured from ovarian carcinoma tumor tissue fragments formed spheroids and succeeded in creating a ductal structure similar to the in vivo structure. We succeeded in forming spheroids derived from ovarian cancer tumor tissue.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2015~2017
課題番号 : 15K10730
研究分野 : Oncology
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